學術及儀器事務處 Academic Affairs and Instrument Service 學術及儀器事務處 Academic Affairs and Instrument Service

Metabolomics Core Facility

　　Following genomics, transcriptomics, and proteomics, metabolomics is a powerful tool considered to be the next step for a comprehensive illustration of biological pathways in organisms. The metabolic profile or expression levels of metabolites reflect final physiological state in response to biotic and abiotic stimulation. Moreover, metabolites act as messengers intimately entwined with cell signaling between individuals and species. Therefore, increased attention has been devoted to using metabolomics for the qualification and quantification of small molecules. Since its founding in 2009, the Academia Sinica Metabolomics Core Facility (ASMCF) has supported the demand for metabolomics analyses, including services for mass spectrometry-based metabolomics analysis. In addition to targeted and predefined candidates, untargeted analyses are provided (also referred to as global metabolite profiling). With advanced instruments, such as liquid chromatography coupled with high resolution-mass spectrometry, we are able to provide a metabolite profile with high accuracy and sensitivity even in a complex biological sample.

Location: Room A523, Agricultural Technology Building

Facility Supervisor: Dr. Yet-Ran Chen, Agricultural Biotechnology Research Center

Managers: Dr. Chia-Wei Hsu and Dr. Kai-Ting Fan, Agricultural Biotechnology Research Center

MS Technicians: Ms. Ya-Wen Chou and Ms. Kuo-Hsin Wang, Agricultural Biotechnology Research Center

Phone Numbers: 2787-2112 (Lab); 2787-2049 (Office)

Website : http://msomics.abrc.sinica.edu.tw/ASMCF/

Availability: Services available to Academia Sinica faculty members and their associates, as well as research institutes and biotech companies outside AS.

Services:

1. Targeted Quantitation
2. Untargeted Metabolomics Analysis (For metabolome profiling, marker identification or pathway mapping)
3. Compound Identification

Equipment:

• Mass Spectrometry
  1. Tribrid Mass Spectrometer (Orbitrap Fusion Lumos, Thermo Scientific)
  2. Hybrid Ion Trap-Orbitrap Mass Spectrometer (Orbitrap Elite, Thermo Scientific)
  3. Dual- pressure Ion Trap Mass Spectrometer (Velos Pro, Thermo Scientific)
• Sample inlet system
  1. Ultra Performance Liquid Chromatography
     • - Waters ACQUITY UPLC System
     • - Thermo Scientific Vanquish UHPLC system
     • - Thermo Scientific Vanquish Horizon UHPLC system
  2. Nano-Ultra Performance Liquid Chromatography
     • - Thermo Scientific UltiMate 3000 RSLCnano system