Neuroscience Core Facility
The Neuroscience Core Facility was established to empower scientists in achieving their goals through technical support and services.
The Neuroscience Core Facility consists of “electrophysiology” and “imaging” facilities. The electrophysiological facilities are mainly used to perform biopsies on animal brain tissue and record various electrophysiological and image signals. This core facility also provides services for live tissue culturing, instrument operation, and image capture, as well as consultations on relevant information. The confocal microscope provides advanced fluorescence image acquisition and relevant analysis software.
For each facility, one-on-one teaching services are provided. The electrophysiology facility is also available to academic units outside AS on an application basis. We welcome scholars from AS and outside AS to use our facilities.
Location:Room I-07, B2 Floor, Interdisciplinary Research Building of Science and Technology
Managers:Dr. Sin-Jhong Cheng, Associate Research Specialist, Institute of Biomedical Sciences (Electrophysiology); Dr. Ya-Jen Cheng, Assistant Research Specialist, Institute of Molecular Biology (Imaging)
Contact Person:Ms. Huei-Fan Wu, Research Assistant, Institute of Biomedical Sciences
Phone Numbers:
- 2789-9343 (Dr. Sin-Jhong Cheng);
- 2787-3281 (Dr. Ya-Jen Cheng);
- 2789-9343 (Ms. Huei-Fang Wu)
Website: https://npas.programs.sinica.edu.tw/core_facility/en/index.html
Availability: Services available to Academia Sinica faculty members as well as academic institutions outside AS.
Services:
- Field-EPSPs recording
- Multi-electrode fEPSP recording in hippocampal slices
- Whole-cell patch-clamp recording
- Calcium imaging recording
- Confocal imaging acquisition
- Imaging recording of Voltage-Sensitive Dye
Equipment:
- Upright microscope (Luigs & Neumann (LN), Scientifica system) for electrophysiological recording.
- MED64 system, a microelectrode array system for in vitro extracellular electrophysiology
- Inverted microscope (Zeiss AxioVert AI system) for whole-cell patch-clamp recording
- Zeiss observer. D1 system for Calcium imaging recording
- Zeiss LSM880 Confocal microscope with Airyscan and incubation system.
- MiCAM05 Neuro Imaging system
- D.S.K. microslicer DTK-1000 Vibrotome
- SUTTER MODEL P-97 Horizontal Pipette puller
- NARISHIGE PC-10 Glass Pipette puller
- Micro-Osmometer Model 3300
- IMARIS image software
Neuroscience Core Facility