Transgenic Core Facility
The Transgenic Core Facility (TCF) was founded in 1997 with support from Academia Sinica and the AS Institute of Molecular Biology. At the TCF, gene-modified (GM) mice are produced using highly advanced technology, including TALEN, CRISPR/Cas9-mediated genome editing. Through accelerating GM mice production, TCF enhances research efficiency for researchers both in industry and academia, giving them a head start in publishing research results. Services are currently being expanded to research institutes outside AS, in order to support science research in Taiwan.
Location: Room N511, Institute of Molecular Biology
Manager: Dr. Ching-Yen Tsai, Associate Research Specialist, Institute of Molecular Biology
Contact Persons: Ms. Lu-Yah Lu and Ms. Mei-Ling Chang, Research Assistants, Institute of Molecular Biology
Phone Number: 2789-9312
Website: http://www.imb.sinica.edu.tw/TCF/
Availability: Services available to Academia Sinica faculty members as well as academic units outside AS.
Services:
- Transgenic mice production
- Genome edited mice using TALEN, CRISPR/Cas9 system
- Gene targeting in ES cells
- Cre recombination in ES
- Chimeric mice production
- Embryo Cryo-preservation
- Sperm Cryo-preservation
- In-vitro Fertilization (IVF)
- Embryo transfer
- Providing tool mice (Cre, Flpe mice)
- Consultation on relevant research designs
Equipment:
- Electroporator
- Microinjection Systems
- Needle Puller
- Microforge
Transgenic Core Facility